Môi trường nuôi cấy MSC MesenCult™-ACF Culture Kit

Môi trường nuôi cấy MSC MesenCult™-ACF Culture Kit

Overview

The MesenCult™-ACF Culture Kit is a standardized, animal component-free and serum-free system for the isolation and culture of human mesenchymal stem cells (MSCs). It consists of MesenCult™-ACF Medium (Catalog #05440) and MesenCult™-ACF Attachment Substrate (Catalog #05444) and is optimized for the expansion of MSCs in vitro as well as their enumeration using the colony-forming unit-fibroblast (CFU-F) assay. This product supports the long-term growth of human bone marrow- and adipose tissue-derived MSCs as cells maintain robust multi-lineage differentiation potential. MesenCult™-ACF Medium must be used in conjunction with the MesenCult™-ACF Attachment Substrate and the MesenCult™-ACF Dissociation Kit (Catalog #05426). Components of the MesenCult™-ACF Attachment Substrate and the MesenCult™-ACF Dissociation Kit are pre-screened and tested for optimal cell adherence when cells are cultured with MesenCult™-ACF Medium. MesenCult™-ACF Medium must be supplemented with L-glutamine.

Advantages:

• Defined, animal component-free culture system
• Optimized for MSC isolation from primary bone marrow and adipose tissue
• Superior cell expansion compared to serum-containing medium
• MSC cultures display robust self-renewal and differentiation potential after long-term passaging
• No adaptation required when transitioning MSCs from serum-containing medium

Components:    

MesenCult™-ACF Culture Kit (Catalog #05449)

MesenCult™-ACF Basal Medium, 400 mL (Catalog #05451)

MesenCult™-ACF 5X Supplement, 100 mL (Catalog #05452)

MesenCult™-ACF Attachment Substrate, 1 mL (Catalog #05444)

Subtype:              Specialized Media

Cell Type:            Mesenchymal Stem and Progenitor Cells

Species:               Human

Application:          Cell Culture; Colony Assay; Expansion

Brand:                  MesenCult

Area of Interest:   Stem Cell Biology

Formulation:        Animal Component-Free; Defined; Serum-Free